Tthssb
WebAug 10, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the … http://www.100md.com/html/DirDu/2006/07/27/12/18/65.htm
Tthssb
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WebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately … WebAug 8, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand
WebNov 15, 2003 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus. TthSSB was also able to bind single-stranded RNA, … WebOct 1, 2002 · Europe PMC is an archive of life sciences journal literature.
WebOct 1, 2002 · TthSSB and TaqSSB monomer proteins contain 264 and 266 amino acids, respectively.The homology between these proteins is very high—82% identity and 90% … WebJun 21, 2006 · In a complementation assay in Escherichia coli, DgeSSB took over the in vivo function of EcoSSB. DgeSSB is thermostable with half-lives of 50 min at 70 degrees C and 5 min at 90 degrees C. Hence, DgeSSB offers an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and for analytical purposes.
WebJul 27, 2006 · TthSSB-255 could only enhance RCA reactions drastically, which showed weak ssDNA binding affinity. This characteristic of the protein would be critical for reduction of nonspecific products. As shown in Supplementary Figure SP2, TthSSB has a significant cluster consisting of acidic residues in its C-terminus although the role has not been …
WebThermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA specifically over a temperature range of 4–80oC, and the wild-type protein could withstand incubation at 94oC for 2 min. Addition of TthSSB to PCR halved the elonga-tion time required for the DNA polymerases of cost plus world market farmington uthttp://www.diva-portal.org/smash/record.jsf?pid=diva2:658627 breast cancer high socksWebJun 21, 2006 · It is shown that DgeSSB is similar to Thermus/Deinococcus SSB in its biochemical properties and offers an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and for analytical purposes. To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus … cost plus world market falls church vaWebOct 15, 2010 · The results showed that TmaSSB and TneSSB are the most thermostable SSB proteins identified to date, offering an attractive alternative to TaqSSB and TthSSB in … cost plus world market floor lampsWebMay 12, 2009 · Use of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500x. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over … cost plus world market flatwareWebDespite this, the ®delity of the Tth DNA polymerase in the presence of TthSSB in these assays was an order of magnitude lower than that obtained with the proof-reading … cost plus world market flagstaff azWebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not … cost plus world market floor cushion